Pregnancy Test The potential of fluorescent Pseudomonas spp.to control Alternaria leaf spot on castorbean, caused by Alternaria ricini, was studied under greenhouse conditions.Two periods for antagonist applications were tested: 48h before and simultaneously to the pathogen inoculation.Among the antagonists tested JA4 and BJ22 were the most effectives showing disease severity reduction of 20.9% and 17.
8% respectively, when applied simultaneously.The effect of Pseudomonas spp.on the micelial growth and sporulation was also studied throughout three different methods (funel, streak and celophane).Inhibition of micelial growth and sporulation was Hydro 4 Parts observed.There was no correlation between in vitro and in vivo data.
Antibiosis was showed as a mode of action for Pseudomonas spp.in relation to Alternaria ricini.Ultrastructural studies confirmed the inhibition of spore germination by the bacteria.